EASY is a mammalian coacErvAte-baSed drug deliverY technology platform, designed for the efficient and targeted delivery of nucleic acids, proteins, small molecules, and other therapeutic agents into cells. EASY is engineered from mammalian proteins capable of forming coacervates, which allow for the encapsulation, protection, and intracellular delivery of therapeutic molecules. Unlike traditional delivery methods, EASY employs a non-viral, non-electroporation, and non-liposomal approach, ensuring optimal biocompatibility, high delivery efficiency, and minimal cytotoxicity. By leveraging the unique properties of endogenous coacervate-forming proteins, EASY enables precise intracellular delivery across a wide range of cell types, including primary immune cells, without the need for harsh physical or chemical treatments. Beyond its applications in ex vivo cell engineering, EASY is also designed for in vivo delivery, offering a safe and effective method for systemic or localized therapeut
EASY平台利用哺乳动物内源蛋白凝聚体的原理形成紧凑、均一的纳米颗粒。这些纳米颗粒可以高效装载数千个核酸分子,确保高装载量和高效递送。该过程包含以下五个步骤:
EASY与核酸自组装,形成稳定的纳米颗粒
纳米颗粒通过非内吞途径高效转导入细胞
进入细胞后,蛋白凝聚体发生自然降解,无残留细胞毒性
包封的核酸在细胞质中快速释放,确保高效递送
释放的核酸进行转录翻译,实现稳定和持续的基因表达
ProteanFect能够成功将mRNA递送到多种原代细胞中,包括T细胞、NK细胞、干细胞、神经元、心肌细胞等
ProteanFect形成的颗粒可包裹数十万个核酸分子,显著超过脂质纳米颗粒的载量容量
与电穿孔相比,ProteanFect在原代细胞中实现更高的转染效率和更好的细胞存活率,确保安全性和最佳性能
ProteanFect能够成功实现多孔板中各种mRNA分子的转染,非常适合大规模基因研究中的高通量应用