Genetic engineering enables T cells to effectively treat a variety of diseases, including cancer and autoimmune disorders. However, transfecting T cells often presents significant challenges, especially in complex research involving multiple genes and targets. The ProteanFect™ Max Transfection Kit offers a non-viral, non-electroporation, and non-liposomal transfection system utilizing engineered mammalian proteins. It can efficiently, safely, and conveniently deliver genes into human primary T cells, making it particularly suitable for the delivery of large and multiple gene fragments.
This protocol provides a detailed introduction on how to use ProteanFect™ Max Transfection Kit (PT02) to achieve efficient transfection of human primary T cells.
PT02 Transfection Protocol for Human Primary T Cells.pdf