ProteanFect™ Max Mouse Immunocyte Transfection Protocol for Mouse Primary T Cells

As immunological research advances, there is an increasing demand for studies involving animals with fully functional immune systems, particularly in the fields of CAR-T cell therapy and autoimmune disease research. However, transfecting mouse primary T cells remains challenging, especially in experiments involving multiple genes and targets. The ProteanFect™ Max Mouse Immunocyte Transfection Kit offers a non-viral, non-electroporation, and non-liposomal transfection system utilizing engineered mammalian proteins. This innovative design achieves high transfection efficiency while maintaining a superior safety profile. The ProteanFect™ Max Mouse Immunocyte Transfection Kit is specifically designed for efficient delivery, particularly suitable for multiple gene fragments into primary mouse immune cells.

 This protocol provides a detailed guide on using the ProteanFect™ Max Mouse Immunocyte Transfection Kit (PT03) to achieve efficient transfection in mouse primary T cells.

PT03 Transfection Protocol for Mouse Primary T Cells.pdf