EASY is a mammalian coacErvAte-baSed drug deliverY technology platform, designed for the efficient and targeted delivery of nucleic acids, proteins, small molecules, and other therapeutic agents into cells. EASY is engineered from mammalian proteins capable of forming coacervates, which allow for the encapsulation, protection, and intracellular delivery of therapeutic molecules. Unlike traditional delivery methods, EASY employs a non-viral, non-electroporation, and non-liposomal approach, ensuring optimal biocompatibility, high delivery efficiency, and minimal cytotoxicity. By leveraging the unique properties of endogenous coacervate-forming proteins, EASY enables precise intracellular delivery across a wide range of cell types, including primary immune cells, without the need for harsh physical or chemical treatments. Beyond its applications in ex vivo cell engineering, EASY is also designed for in vivo delivery, offering a safe and effective method for systemic or localized therapeut
The EASY platform utilizes a mammalian coacervate-based approach to form compact, uniform nanoparticles with molecules. For instance, these nanoparticles encapsulate hundreds of thousands of nucleic acids, ensuring high cargo loading capacity and efficient delivery. The process follows five key steps:
Mammalian proteins self-assemble with nucleic acids, forming stable nanoparticles.
The nanoparticles efficiently internalize into cells via endocytosis-free mechanism.
The encapsulated nucleic acids are quickly released into the cytoplasm, ensuring functional delivery.
The released genetic material undergoes translation, enabling robust and sustained gene expression.
Once the delivery process is complete, the conservates naturally degrade, leaving no artificial materials or toxicity.
ProteanFect enables the successful delivery of mRNA into a wide range of primary cells, including T cells, NK cells, stem cells, neurons, cardiomyocytes, and more
ProteanFect-formed particles encapsulate hundreds of thousands of nucleic acid molecules, significantly exceeding the payload capacity of lipid nanoparticles
ProteanFect achieves higher transfection efficiency and better cell viability in primary cells compared to electroporation, ensuring both safety and optimal performance
ProteanFect facilitates the successful transfection of multiple wells with various mRNA molecules, making it ideal for high-throughput applications in large-scale genetic studies